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Interphase fluorescent in situ hybridization deletion analysis of the 9p21 region and prognosis in childhood acute lymphoblastic leukaemia (ALL): results from a prospective analysis of 519 Nordic patients treated according to the NOPHO‐ALL 2000 protocol
Author(s) -
Kuchinskaya Ekaterina,
Heyman Mats,
Nordgren Ann,
Söderhäll Stefan,
Forestier Erik,
Wehner Peder,
Vettenranta Kim,
Jonsson Olafur,
Wesenberg Finn,
Sahlén Sigrid,
Nordenskjöld Magnus,
Blennow Elisabeth
Publication year - 2011
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.2010.08532.x
Subject(s) - fluorescence in situ hybridization , interphase , hematology , cytogenetics , biology , incidence (geometry) , medicine , immunology , genetics , gene , chromosome , physics , optics
Summary Interphase fluorescent in situ hybridization (FISH) was applied on diagnostic BM smears from 519 children with acute lymphoblastic leukaemia (ALL) in order to establish the frequency and prognostic importance of 9p21 deletion in children enrolled in the Nordic Society of Paediatric Haematology and Oncology (NOPHO) – 2000 treatment protocol. Among the patients, 452 were diagnosed with B‐cell precursor (BCP)‐ALL and 66 with T‐ALL. A higher incidence of 9p21 deletions was found in T‐ALL (38%) compared to BCP‐ALL (15·7%). Homozygous deletions were found in 19·7% of T‐ALL and 4·0% of BCP‐ALL; hemizygous deletions were found in 18·2% and 11·7% respectively. In our series, 9p21 deletions were detected in all age groups with a steady rise in the frequency with age. There was no significant difference in outcome between cases with or without 9p21 deletion or between cases with hemi‐ or homozygous deletions of 9p21. In conclusion, in this large series of childhood ALL deletion of 9p21 was not associated with worse prognosis. However, interphase FISH deletion analysis of 9p21 could be used as a first step to detect unfavourable subtle cytogenetic aberrations such as the dic(9;20) rearrangement.