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Purification of FANCD2 sub‐complexes
Author(s) -
Zhi Gang,
Chen Xiaoyong,
Newcomb William,
Brown Jay,
Semmes Oliver J.,
Kupfer Gary M.
Publication year - 2010
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.2010.08217.x
Subject(s) - fancd2 , fanconi anemia , tetramer , dna , mlh1 , genetics , biology , chemistry , microbiology and biotechnology , dna repair , biochemistry , dna mismatch repair , enzyme
Summary Fanconi anaemia (FA) is a recessive genetic disorder characterized by bone marrow failure, birth defects and cancer. Cells from FA patients are particularly defective in removing DNA interstrand crosslinks. We have developed a working chromatography purification scheme for FANCD2, a pivotal player in the FA DNA repair pathway, to facilitate identification of FANCD2 interacting partners. In doing so, at least three distinct FANCD2 subcomplexes were found to be present, designated as large, middle, and small complexes. The small complex is composed of tetramer of FANCD2 polypeptides, which may be the building block for other FANCD2 subcomplexes.