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Multipotent adult progenitor cell isolation and proliferation in cytokine and serum‐free medium conditioned by rat B104 cells
Author(s) -
Rice Claire M.,
Clavel Carlos,
Mazo Manu,
Prosper Felipe,
Scolding Neil J.
Publication year - 2010
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.2009.07986.x
Subject(s) - progenitor cell , microbiology and biotechnology , cytokine , progenitor , biology , immunology , chemistry , stem cell
Summary This report describes the isolation of rodent multipotent adult progenitor cells (MAPCs) and proliferation of these cells in both standard medium and medium without exogenous serum or growth factors conditioned by the rat cell line B104. MAPCs have exacting requirements for their proliferation in vitro but once established proliferate rapidly at low seeding density, requiring almost daily passage and media exchange. Previously published methods for growth of MAPCs in vitro all used media supplemented with serum and growth factors, which adds considerable expense.