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Comparison of miRNA profiles of microdissected Hodgkin/Reed‐Sternberg cells and Hodgkin cell lines versus CD77 + B‐cells reveals a distinct subset of differentially expressed miRNAs
Author(s) -
Van Vlierberghe Pieter,
De Weer An,
Mestdagh Pieter,
Feys Tom,
De Preter Katleen,
De Paepe Pascale,
Lambein Kathleen,
Vandesompele Jo,
Van Roy Nadine,
Verhasselt Bruno,
Poppe Bruce,
Speleman Frank
Publication year - 2009
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.2009.07909.x
Subject(s) - reed–sternberg cell , microrna , biology , cell culture , microbiology and biotechnology , cancer research , lymphoma , genetics , gene , immunology , hodgkin lymphoma
Summary Classical Hodgkin lymphoma (cHL) is characterized by the presence of malignant Hodgkin and Reed Sternberg (HRS) cells. The scarcity of tumour cells in lymphoma biopsies has hampered genetic analyses of HRS cells, including microRNA (miRNA) expression profiling. We determined the expression of 360 miRNAs in microdissected HRS cells from nine cHL patients. These miRNA profiles were compared to those from four cHL cell lines and CD77 + B‐cells, yielding a distinct cHL signature of 12 over‐ and three underexpressed miRNAs. Our data suggest that miRNAs are implicated in the pathogenesis of Hodgkin lymphoma and prompt further investigations concerning their role in cHL.