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Fluorescence‐activated cell sorting (FACS) of whole mount in situ hybridization (WISH) labelled haematopoietic cell populations in the zebrafish
Author(s) -
Dobson Joseph T.,
Da’as Sahar,
McBride Eileen R.,
Berman Jason N.
Publication year - 2009
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.2008.07527.x
Subject(s) - zebrafish , cell sorting , in situ hybridization , biology , haematopoiesis , fluorescence in situ hybridization , microbiology and biotechnology , embryonic stem cell , cell , stem cell , messenger rna , genetics , gene , chromosome
Summary The zebrafish is a robust animal model for studying vertebrate haematopoiesis and immune cell interactions. However, fluorescence activated cell sorting (FACS) has been limited due to a paucity of available functional zebrafish antibodies. We have developed a technique combining FACS with whole mount in situ hybridization (WISH) that enables the sorting and examining of fixed zebrafish blood cell populations at different stages of embryonic development, providing the opportunity to correlate RNA expression data with cellular morphology.