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Multiplex ligation‐dependent probe amplification for detection of genomic alterations in chronic lymphocytic leukaemia
Author(s) -
CollMulet Llorenç,
Santidrián Antonio F.,
Cosialls Ana M.,
IglesiasSerret Daniel,
De Frias Mercè,
Grau Javier,
Menoyo Anna,
GonzálezBarca Eva,
Pons Gabriel,
Domingo Alicia,
Gil Joan
Publication year - 2008
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.2008.07268.x
Subject(s) - multiplex ligation dependent probe amplification , multiplex , fluorescence in situ hybridization , biology , chromosome , genetics , gene , exon
Summary Chronic lymphocytic leukaemia (CLL) is the commonest form of leukaemia in adults in Western countries. We performed multiplex ligation‐dependent probe amplification (MLPA) analysis in 50 CLL patients to identify multiple genomic CLL‐specific targets, including genes located at 13q14, 17p13 ( TP53 ), 11q23 ( ATM ) and chromosome 12, and compared the results with those obtained with fluorescence in situ hybridization (FISH). There was a good correlation between MLPA and FISH results, as most alterations (89%) were detected by both techniques. Only three cases with a low percentage (<25%) of cells carrying the alterations were not detected by MLPA. On the other hand, as MLPA uses multiple probes it identified intragenic or small alterations undetected by FISH in three cases. MLPA also detected alterations in 8q24 ( MYC ) and 6q25–26. In summary, unlike interphase FISH, MLPA enabled the simultaneous analysis of many samples with automated data processing at a low cost. Therefore, the combination of robust multiplexing and high throughput makes MLPA a useful technique for the analysis of genomic alterations in CLL.