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Fibroblast activation protein α identifies mesenchymal stromal cells from human bone marrow
Author(s) -
Bae Sohyun,
Park Chae Woon,
Son Hye Kyung,
Ju Hye Kyung,
Paik Donggi,
Jeon ChoonJu,
Koh Gou Young,
Kim Jaeseob,
Kim Hoeon
Publication year - 2008
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.2008.07241.x
Subject(s) - fibroblast activation protein, alpha , mesenchymal stem cell , stromal cell , bone marrow , fibroblast , biology , cell , cancer research , microbiology and biotechnology , immunology , cell culture , biochemistry , genetics , cancer
Summary Mesenchymal stromal cells (MSCs) have gained widespread popularity in cell therapy, but their development into clinical products has been impeded by the scarcity of cell‐specific markers. We previously explored transcriptome and membrane proteome of MSCs, from which fibroblast activation protein α (FAP) was recognized as a prime surface marker candidate. The present study showed that FAP was constitutively expressed on MSCs, but not on other cells. FAP immunoselection yielded homogeneous MSCs from cryopreserved bone marrow (BM). These results suggest that FAP serves as a surface protein marker that can singly define MSCs from BM and possibly from other sources.