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Intentional induction of mixed haematopoietic chimerism as platform for cellular therapy after HLA‐matched allogeneic stem cell transplantation in childhood leukaemia patients
Author(s) -
Pérez Antonio,
GonzálezVicent Marta,
Ramirez Manuel,
Sevilla Julián,
Madero Luis,
Díaz Miguel Angel
Publication year - 2008
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.2007.06911.x
Subject(s) - medicine , stem cell , haematopoiesis , transplantation , cd34 , immunology , myeloid , donor lymphocyte infusion , human leukocyte antigen , hematopoietic stem cell transplantation , leukemia , hematology , gastroenterology , antigen , biology , genetics
Summary Allogeneic peripheral blood stem cell CD34 + ‐selected transplantation followed by donor lymphocyte infusion (DLI) to maximize graft‐versus‐leukaemia effect while avoiding graft‐versus‐host disease was investigated in 22 paediatric patients with acute myeloid leukaemia ( n = 10) or acute lymphoblastic leukaemia ( n = 12). Patients were grafted with a median (range) 6 × 10 6 (2–31 × 10 6 )/kg CD34 + cells and 1·1 × 10 4 (0·2–3·9 × 10 4 ) CD3 + cells. Seventy‐five DLI were performed with no complications. Median time (range) to neutrophil and platelet engraftment was 13 (11–15) and 12 d (8–13) respectively. Probability of relapse and disease‐free survival was 23 ± 9% and 72 ± 6% respectively (median follow‐up of 15 months).