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Distribution of t(14;18)‐positive, putative lymphoma precursor cells among B‐cell subsets in healthy individuals 1
Author(s) -
Hirt Carsten,
Dölken Gottfried,
Janz Siegfried,
Rabkin Charles S.
Publication year - 2007
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.2007.06671.x
Subject(s) - lymphoma , distribution (mathematics) , immunology , medicine , biology , mathematics , mathematical analysis
Summary The t(14;18)(q32;q21) is the characteristic chromosomal translocation of follicular lymphoma (FL). Highly sensitive polymerase chain reaction (PCR) techniques can also detect t(14;18)‐sequences in the blood and lymphoid tissues of healthy individuals (HI). The aim of this study was to determine the immunophenotypic markers of t(14;18)‐positive cells in HI and to relate these features to lymphocyte maturation. B cells from 10 subjects with t(14;18)‐positive and three subjects with t(14;18)‐negative peripheral blood mononuclear cells (PBMC) were fluorescence‐activated cell sorted for antigen‐naïve (CD27 − ), immunoglobulin M (IgM) memory (IgM + CD27 + ) and switched memory (IgM − CD27 + ) cells. t(14;18)‐recombinations were detected by quantitative PCR. Among PBMC‐positive subjects, t(14;18)‐frequency was significantly higher in IgM memory (median: 380/10 6 ) than in antigen‐naïve (median: 16/10 6 ) or switched memory (median: 5/10 6 ) B cells. All PBMC‐negative subjects nevertheless had detectable t(14;18) in sorted B cells; levels were lower than in PBMC‐positive subjects, but had the same relative predominance. These results suggest that t(14;18) is generated during early B‐cell development in the bone marrow and that affected cells may mature and expand in germinal centres. t(14;18)‐frequency was highest in IgM memory cells, a B‐cell subset that shares immunophenotypic similarities with FL. The significance of these cells as lymphoma precursors or indicators of lymphoma risk remains to be established.