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Thrombin generation: the functional role of alpha‐2‐macroglobulin and influence of developmental haemostasis
Author(s) -
Ignjatovic V.,
Greenway A.,
Summerhayes R.,
Monagle P.
Publication year - 2007
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.2007.06663.x
Subject(s) - alpha 2 macroglobulin , thrombin generation , macroglobulin , thrombin , chromogenic , alpha (finance) , chemistry , medicine , immunology , platelet , chromatography , surgery , construct validity , patient satisfaction
Summary Currently available chromogenic and fluorogenic substrates for endogenous thrombin potential (ETP) measurement are cleaved by both free (active) and alpha‐2‐macroglobulin‐bound (inactive) thrombin, leading to an overestimation of ETP. Commercial methods for ETP measurement determine this using a mathematical algorithm, which assumes the contribution of alpha‐2‐macroglobulin to the ETP. This limits application of such methods to populations where variation in alpha‐2‐macroglobulin concentrations is observed, primarily children. This study examined the contribution of alpha‐2‐macroglobulin‐bound thrombin to the ETP measurement in neonates, children and adults, to determine whether automated methods are appropriate for use in neonates and children.