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Human Fanconi A cells are susceptible to TRAIL‐induced apoptosis
Author(s) -
Pigullo Simona,
Ferretti Elisa,
Lanciotti Marina,
Bruschi Maurizio,
Candiano Giovanni,
Svahn Johanna,
Haneline Laura,
Dufour Carlo,
Pistoia Vito,
Corcione Anna
Publication year - 2007
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.2006.06432.x
Subject(s) - fanca , fanconi anemia , apoptosis , haematopoiesis , cancer research , flow cytometry , biology , tumor necrosis factor alpha , cell culture , microbiology and biotechnology , poly adp ribose polymerase , immunology , stem cell , polymerase , genetics , dna repair , dna
Summary Tumour necrosis factor (TNF) contributes to the pathogenesis of bone marrow failure in Fanconi anaemia (FA) patients. The sensitivity of haematopoietic cells from FA, complementation group A (FANCA) subjects, who represent the majority of FA patients, to TNF‐related apoptosis‐inducing ligand (TRAIL) is unknown. The human lymphoblastoid FANCA HSC072 cell line and the genetically corrected counterpart HSC072FANCA‐neo were tested for apoptoptic response to TRAIL using flow cytometry and Western blotting. FANCA cells were more sensitive to TRAIL‐induced apoptosis than their corrected counterparts, indicating that TRAIL negatively regulates haematopoietic FANCA cell lines. This effect involved poly(ADP‐ribose) polymerase‐1 cleavage and caspase‐8 activation.