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Differential cell division history between neutrophils and macrophages in their development from granulocyte–macrophage progenitors
Author(s) -
Sugimoto Yuka,
Katayama Naoyuki,
Masuya Masahiro,
Miyata Eri,
Ueno Masaya,
Ohishi Kohshi,
Nishii Kazuhiro,
Takakura Nobuyuki,
Shiku Hiroshi
Publication year - 2006
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.2006.06367.x
Subject(s) - haematopoiesis , progenitor cell , cell division , biology , granulocyte , microbiology and biotechnology , immunology , bromodeoxyuridine , macrophage , monocyte , cellular differentiation , cell cycle , cell , stem cell , in vitro , genetics , immunohistochemistry , gene
Summary The appearance of monocytes before neutrophils in the blood during haematopoietic recovery in myelosuppressive patients is commonly observed, thus suggesting a difference in the cell division history between these two lineages in the differentiation from granulocyte–macrophage (GM) progenitors. We investigated the cell division histories of murine GM progenitors. When analysed by the dye dilution method, GM progenitors gave rise to Gr‐1 + Fms + and Gr‐1 + Fms − cells that passed through similar rounds of cell division during initial 5 d of culture. The Gr‐1 + Fms + cells showed morphological features of monocytes, while Gr‐1 + Fms − cells exhibited an immature morphology of neutrophils. In the subsequent culture, a decline in the number of Gr‐1 + Fms + cells was observed, while Gr‐1 + Fms − cells increased. The proliferation of Gr‐1 + Fms − cells and no cell division of Gr‐1 + Fms + cells were confirmed by DNA staining, Ki‐67 expression, membrane dye staining and bromodeoxyuridine incorporation. These Gr‐1 + Fms − cells acquired mature neutrophil morphology, whereas Gr‐1 + Fms + cells became macrophages. These results demonstrate that GM progenitors generate postmitotic monocytes earlier than mature neutrophils. Our data may also offer one explanation for the rapid recovery of monocytes in comparison with neutrophils in the early phase of haematopoietic regeneration.