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Globin gene expression in Hb Lepore‐BAC transgenic mice
Author(s) -
SloaneStanley Jackie,
Roberts Nigel A.,
Olivieri Nancy,
Weatherall David J.,
Wood William G.
Publication year - 2006
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.2006.06354.x
Subject(s) - transgene , biology , gene , genetically modified mouse , context (archaeology) , genetics , fusion gene , gene expression , microbiology and biotechnology , gene cluster , globin , paleontology
Summary We generated five lines of transgenic mice carrying 1–3 copies of the Hb Lepore δ β fusion gene, in the context of a Bacterial Artificial Chromosome containing the whole human β globin gene cluster. Normal developmental regulation of human genes occurred at levels approximating to those of endogenous genes. δ β transgene expression became detectable during fetal life and rose to a mean level of 13·0% in adults, similar to that of humans. Low levels of human γ chains were detectable as F cells in adult mice, but numbers did not increase after treatment with drugs that raise F cells in human subjects, even on a thalassaemic background.