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Resolution of ambiguous low‐level positive quantitative polymerase chain reaction results in TEL‐AML1 positive ALL using a post‐PCR fluorescent oligoligation method *
Author(s) -
Chen IMing,
Chakerian Artemis,
Devidas Meenakshi,
Borowitz Michael J.,
Hunger Stephen P.,
Willman Cheryl L.,
Viswanatha David S.
Publication year - 2006
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.2006.06320.x
Subject(s) - minimal residual disease , polymerase chain reaction , real time polymerase chain reaction , primer (cosmetics) , microbiology and biotechnology , medicine , biology , immunology , chemistry , genetics , leukemia , gene , organic chemistry
Summary The interpretation of low‐level or non‐reproducible amplification results in clinical quantitative polymerase chain reaction (Q‐PCR) assays can be difficult to definitively resolve. Concerning minimal residual disease detection in leukaemia, indeterminate low‐level results might create prognostic or therapeutic dilemmas. We evaluated low‐level, ambiguous Q‐PCR results in a study of paired diagnostic and end‐induction (day 29) TEL‐AML1 positive acute lymphoblastic leukaemia samples utilising a novel fluorescent primer ligation detection assay. The data presented here indicate that a significant number of low‐level apparent Q‐PCR positive results may be spurious or non‐specific in nature, requiring additional technical manoeuvres for confirmation of true positive cases.