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Differential gene expression in non‐malignant tumour microenvironment is associated with outcome in follicular lymphoma patients treated with rituximab and CHOP
Author(s) -
Harjunpää Antti,
Taskinen Minna,
Nykter Matti,
KarjalainenLindsberg MarjaLiisa,
Nyman Heidi,
Monni Outi,
Hemmer Samuli,
YliHarja Olli,
Hautaniemi Sampsa,
Meri Seppo,
Leppä Sirpa
Publication year - 2006
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.2006.06255.x
Subject(s) - follicular lymphoma , rituximab , chop , lymphoma , medicine , cancer research , follicular phase , oncology , immunology
Summary Rituximab in combination with chemotherapy (immunochemotherapy) is one of the most effective treatments available for follicular lymphoma (FL). This study aimed to determine whether differences in gene expression in FL tissue correlate with outcome in response to rituximab and CHOP (cyclophosphamide, doxorubicin, vincristine, prednisone) chemotherapy (R–CHOP). We divided 24 patients into long‐ [time to treatment failure (TTF) >35 months] and short‐term (TTF <23 months) responders, and analysed the gene expression profiles of lymphoma tissue using oligonucleotide microarrays. We used a supervised learning technique to identify genes correlating with outcome, and confirmed the expression of selected genes with quantitative polymerase chain reaction (qPCR) and immunohistochemistry. Among the transcripts with a high correlation between microarray and qPCR analyses, we identified EPHA1 , a tyrosine kinase involved in transepithelial migration, SMAD1 , a transcription factor and a mediator of bone morphogenetic protein and transforming growth factor‐ β signalling, and MARCO , a scavenger receptor on macrophages. According to Kaplan–Meier estimates, high EPHA1 , and low SMAD1 and MARCO expression were associated with better progression‐free survival (PFS). Immunohistochemistry showed that EphA1 was primarily localised in granulocytes. In addition, both EphA1 and Smad1 were expressed in vascular endothelia. However, no difference in vasculature was detected between long‐ and short‐term responders. In a validation set of 40 patients, a trend towards a better PFS was observed among patients with high EphA1 expression. We conclude that gene expression in non‐malignant cells contributes to clinical outcome in R–CHOP‐treated FL patients.