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Reversal of immune thrombocytopenia in mice by cross‐linking human immunoglobulin G with a high‐affinity monoclonal antibody
Author(s) -
Bazin Renée,
Lemieux Réal,
Tremblay Tony
Publication year - 2006
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.2006.06245.x
Subject(s) - monoclonal antibody , antibody , immunology , immune system , thrombocytopenic purpura , in vivo , monoclonal , immunoglobulin g , ex vivo , medicine , biology , microbiology and biotechnology
Summary Intravenous immunoglobulins (IVIgs) are used to treat an increasing number of autoimmune diseases, but their exact mechanism of action remains unknown. This study showed that cross‐linking of human IgG present in IVIg preparations using a mouse monoclonal anti‐human IgG generated complexes that prevented or reversed thrombocytopenia in mice more efficiently than IVIg. Furthermore, biologically active complexes were obtained simply by adding the monoclonal antibody to human serum. These results suggest the possible development of an IVIg‐free substitute through the ex vivo , and possibly in vivo , formation of immune complexes containing autologous IgG of immune thrombocytopenic purpura patients.