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Safe adoptive transfer of virus‐specific T‐cell immunity for the treatment of systemic adenovirus infection after allogeneic stem cell transplantation
Author(s) -
Feuchtinger Tobias,
MatthesMartin Susanne,
Richard Celine,
Lion Thomas,
Fuhrer Monika,
Hamprecht Klaus,
Handgretinger Rupert,
Peters Christina,
Schuster Friedhelm R.,
Beck Robert,
Schumm Michael,
Lotfi Ramin,
Jahn Gerhard,
Lang Peter
Publication year - 2006
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.2006.06108.x
Subject(s) - adoptive cell transfer , immunology , transplantation , cd8 , t cell , stem cell , immunity , medicine , virus , immunosuppression , immune system , virology , biology , genetics
Summary During periods of immunosuppression, such as postallogeneic stem cell transplantation (SCT), patients are at significant risk for severe viral infections. Human adenovirus (HAdV) infection is a serious complication post‐SCT, especially in children. Virus‐specific T cells are essential for the clearance of HAdV, as antiviral chemotherapy has revealed limited success. We present feasibility data for a new treatment option using virus‐specific donor T cells for adoptive transfer of immunity to patients with HAdV‐infection/reactivation. Virus‐specific donor T cells were isolated and infused into nine children with systemic HAdV infection after SCT. Isolation was based on γ ‐interferon (IFN‐ γ ) secretion after short in vitro stimulation with viral antigen, resulting in a combination of CD4 + and CD8 + T cells. 1·2–50 × 10 3 /kg T cells were infused for adoptive transfer. Isolated cells showed high specificity and markedly reduced alloreactivity in vitro . Adoptive transfer of HAdV‐specific immunity was successful in five of six evaluable patients, documented by a dose‐independent and sustained in vivo expansion of HAdV‐specific T cells, associated with a durable clearance/decrease of viral copies. T‐cell infusion was well tolerated in all nine patients, except one case with graft‐ versus ‐host disease II of the skin. In conclusion, induction of a specific T‐cell response through adoptive transfer was feasible and effective. When performed early in the course of infection, adoptive T‐cell transfer may protect from HAdV‐related complications.