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Expression levels of asparagine synthetase in blasts from children and adults with acute lymphoblastic leukaemia
Author(s) -
Leslie M.,
Case M. C.,
Hall A. G.,
Coulthard S. A.
Publication year - 2006
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.2005.05945.x
Subject(s) - asparagine synthetase , asparagine , peripheral blood mononuclear cell , asparaginase , medicine , myeloid , acute lymphocytic leukemia , peripheral blood , immunology , lymphoblastic leukemia , leukemia , gastroenterology , enzyme , biology , in vitro , genetics , biochemistry
Summary l ‐asparaginase is active in the treatment of acute lymphoblastic leukaemia (ALL) through the depletion of serum asparagine. Here we report that median asparagine synthetase ( AS ) mRNA levels were higher in acute myeloid leukaemia (AML) than ALL blasts in both children and adults, with intermediate levels in normal peripheral blood mononuclear cells (NPBMC). NPBMC versus child ALL (Tukeys multiple comparison test, P < 0·05); child ALL versus child AML ( P < 0·001) and adult ALL versus adult AML ( P < 0·01) were all significant and support the hypothesis that selectivity to treatment with l ‐asparaginase is due, at least in part, to lower AS expression.