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P210 Bcr‐abl tyrosine kinase interaction with histone deacetylase 1 modifies histone H4 acetylation and chromatin structure of chronic myeloid leukaemia haematopoietic progenitors
Author(s) -
Brusa Gianluca,
Zuffa Elisa,
Mancini Manuela,
Benvenuti Michela,
Calonghi Natalia,
Barbieri Enza,
Santucci Maria Alessandra
Publication year - 2006
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.2005.05873.x
Subject(s) - cancer research , biology , histone deacetylase , histone h4 , microbiology and biotechnology , chromatin , chromatin remodeling , histone deacetylase 5 , hdac11 , histone , histone deacetylase 2 , histone h3 , genetics , gene
Summary The BCR‐ABL fusion gene, originating from the balanced (9;22) translocation, is the molecular hallmark and the causative event of chronic myeloid leukaemia (CML). The interactions of its p210 protein constitutively activated and improperly confined to the cytoplasm with multiple regulatory signals of cell cycle progression, apoptosis and self‐renewal, induce the illegitimate enlargement of clonal haematopoiesis and genetic instability that drives its progression towards the fully transformed phenotype of blast crisis. However, its effects on the basic transcription machinery and chromatin remodelling are unknown. Our study underscored histone H4 hyperacetylation associated with p210 tyrosine kinase in vitro and in vivo and its role in BCR‐ABL transcription. Histone H4 hyperacetylation proceeds, at least partly, from the ‘loss of function’ of histone deacetylase 1 protein, a critical component of Rb‐mediated transcriptional repression, in consequence of its cytoplasmatic compartmentalisation.