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Isotype‐specific detection of ABO blood group antibodies using a novel flow cytometric method
Author(s) -
Stussi G.,
Huggel K.,
Lutz H. U.,
Schanz U.,
Rieben R.,
Seebach J. D.
Publication year - 2005
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.2005.05705.x
Subject(s) - abo blood group system , antibody , isotype , immunology , immunoglobulin g , flow cytometry , group a , b cell , haemolysis , immunoglobulin m , hemagglutination , group b , microbiology and biotechnology , biology , medicine , monoclonal antibody
Summary Several methods to detect anti‐A/B antibodies based on haemagglutination and haemolysis have been described. These methods measure predominantly anti‐A/B immunoglobulin (Ig)M, whereas anti‐A/B IgG and IgG subclasses are less well examined. We established a flow cytometry method (ABO‐fluorescence‐activated cell sorting; ABO‐FACS) to quantify binding of anti‐A/B IgM, IgG and IgG subclasses to human A or B red blood cells. Anti‐A/B IgM were present in the majority of 120 blood donors, as expected from blood group typing. The sensitivity and specificity of anti‐A/B IgM to predict the blood group was 93% and 96% respectively. Anti‐A/B IgG was found in 34/38 blood group O samples (89%). Anti‐B IgG in blood group A or anti‐A IgG in blood group B was present in 4/28 (14%) and 1/28 (4%) samples, respectively, and absent in 26 AB sera. IgG2 was the predominant IgG subclass. The correlation of anti‐A/B IgM and IgG in the ABO‐FACS with haemagglutination titres was 0·870 and 0·783, respectively ( n = 240; P < 0·001) whereas the comparison of ABO‐FACS with ABO‐enzyme‐linked immunosorbent assay was less significant. In conclusion, ABO‐FACS is a valid method to quantify anti‐A/B IgM, IgG and IgG subclasses. It opens the possibility of isotype‐specific monitoring of anti‐A/B antibodies levels after ABO‐incompatible solid organ and stem cell transplantation.