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Gene expression profiling of mantle cell lymphoma cells reveals aberrant expression of genes from the PI3K‐AKT, WNT and TGFβ signalling pathways
Author(s) -
Rizzatti Edgar Gil,
Falcão Roberto Passetto,
Panepucci Rodrigo Alexandre,
ProtoSiqueira Rodrigo,
AnselmoLima Wilma Terezinha,
Okamoto Oswaldo Keith,
Zago Marco Antonio
Publication year - 2005
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.2005.05630.x
Subject(s) - biology , gene expression profiling , wnt signaling pathway , pi3k/akt/mtor pathway , mantle cell lymphoma , microarray analysis techniques , microarray , gene expression , gene , cancer research , dna microarray , microbiology and biotechnology , signal transduction , lymphoma , immunology , genetics
Summary Microarray studies have revealed the differential expression of several genes in mantle cell lymphoma (MCL), but it is unknown which of these differences are dependent on the transformed MCL cell itself or on the tumour microenvironment. To investigate which genes and signalling pathways are aberrantly expressed in MCL cells we used oligonucleotide microarrays to perform gene expression profiling of both purified leukaemic MCL cells and their normal counterparts, the naive B cells. A total of 106 genes were differentially expressed at least threefold in MCL cells compared with naive B cells; 63 upregulated and 43 downregulated. To validate the microarray results in a larger set of samples, we selected 10 differentially expressed genes and quantified their expression by real‐time polymerase chain reaction in peripheral blood of MCL patients ( n = 21), purified MCL cells ( n = 6) and naive B cells ( n = 4), obtaining fully concordant results. A computer‐assisted approach was used to procure specific molecular signalling pathways that were aberrantly expressed in MCL cells. Several genes related to apoptosis and to the PI3K/AKT, WNT and tumour growth factor β signalling pathways were altered in MCL cells when compared with naive B cells. These pathways may play a significant role in the pathogenesis of MCL and deserve further investigation as candidates for new therapeutic targets.