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Induction of CCAAT/enhancer binding protein‐δ by cytokinins, but not by retinoic acid, during granulocytic differentiation of human myeloid leukaemia cells
Author(s) -
Ishii Yuki,
Kasukabe Takashi,
Honma Yoshio
Publication year - 2005
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.2004.05326.x
Subject(s) - retinoic acid , myeloid leukaemia , myeloid , ccaat enhancer binding proteins , cellular differentiation , biology , microbiology and biotechnology , enhancer , chemistry , cancer research , dna binding protein , biochemistry , cell culture , genetics , gene , gene expression , transcription factor
Summary Cytokinins, purine derivatives that act as hormones to control many processes in plants, are very effective at inducing the granulocytic differentiation of human myeloid leukaemia cells. Isopentenyladenine (IPA), a potent cytokinin, significantly induced the expression of CCAAT/enhancer‐binding protein (C/EBP) δ , but not C/EBP α protein, whereas all‐ trans retinoic acid, a well‐known inducer of granulocytic differentiation, induced C/EBP α but not C/EBP δ protein. Antisense oligonucleotide for C/EBP δ , but not C/EBP α or C/EBP β , effectively suppressed IPA‐induced differentiation, suggesting that the expression of C/EBP δ protein is necessary for cytokinin‐induced differentiation. Although C/EBP α is known to be crucial for granulocytic differentiation, the function of C/EBP δ has not been well documented in the regulation of haematopoiesis. The role of C/EBP δ in the granulocytic differentiation of myeloid leukaemia cells is discussed.