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A class II‐restricted cytotoxic T‐cell clone recognizes a human minor histocompatibility antigen with a restricted tissue distribution
Author(s) -
Holloway Penny A.,
Kaldenhoven Niels,
KokSchoemaker Henriette M.,
Dijk Mirjam van,
Otten Hennie G.,
Tilanus Marcel,
Postma Saskia,
Mutis Tuna,
Lokhorst Henk M.,
Bloem Andries C.
Publication year - 2005
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.2004.05283.x
Subject(s) - cytotoxic t cell , minor histocompatibility antigen , antigen , immunology , human leukocyte antigen , cd40 , biology , antigen presenting cell , transplantation , t cell , major histocompatibility complex , microbiology and biotechnology , immune system , medicine , genetics , in vitro
Summary Following a human leucocyte antigen (HLA)‐identical allogeneic stem cell transplantation (allo‐SCT), minor histocompatibility antigens (mHags) play an important role in the induction of graft‐ versus ‐leukaemia (GvL) and graft‐ versus ‐myeloma (GvM). Many mHags show ubiquitous tissue expression and are associated with GvL and graft‐ versus ‐host disease. Here we describe a cytotoxic CD4 + T lymphocyte line and a cytotoxic, CD4 + T cell clone (CTC), 3AB11, which recognized a tissue‐restricted mHag. This CTC was isolated from a multiple myeloma patient with clinical GvM following an HLA‐matched allo‐SCT. CTC 3AB11 was activated in a HLA‐DP*0401 restricted fashion and the antigen was expressed by 27% of HLA‐DP*0401 positive Epstein–Barr virus (EBV)‐transformed B‐cell lines (EBV‐B). Tissue distribution analysis of antigen 3AB11 showed it to be expressed by patient‐derived EBV‐transformed B cell lines (EBVp), the myeloma plasma cell‐line UM9 and monocytes. It was weakly expressed by peripheral blood‐derived phytohaemagglutinin‐induced T‐cell blasts and absent on CD40L stimulated peripheral B (CD40L B) cells and stromal cells. The relatively high prevalence of the HLA class II‐restricted 3AB11 antigen, together with its apparent haematopoietic‐restricted expression, makes it an antigen of interest for cellular immunotherapy.