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Factor‐V expression in platelets from human megakaryocytic culture
Author(s) -
Giampaolo Adele,
Vulcano Francesca,
Macioce Giampiero,
Mattia Gianfranco,
Barca Alessandra,
Milazzo Luisa,
Ciccarelli Carmela,
Hassan Hamisa J.
Publication year - 2005
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.2004.05279.x
Subject(s) - platelet , thrombopoiesis , megakaryocyte , immunofluorescence , flow cytometry , microbiology and biotechnology , biology , blot , in vitro , cell culture , platelet factor 4 , progenitor cell , immunology , gene , stem cell , antibody , biochemistry , genetics
Summary The origin of platelet‐factor‐V has long been discussed. To elucidate whether and when human platelet‐factor‐V is synthesized by megakaryocytes, we utilized in vitro ‐generated megakaryocytes capable of producing platelets. Factor‐V gene was silent in purified progenitors and megakaryocytic precursors but was expressed in late culture phase and maintained also in platelets. Similarly, factor‐V protein was expressed in mature proplatelet‐bearing megakaryocytes (immunofluorescence analysis); it was also detectable in cultured megakaryocytes and platelets (Western blotting) and within permeabilized cultured platelets (flow cytometry). The absence of other cells in our culture system indicates conclusively that human megakaryocytes synthesize factor‐V.