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Detection of p53 dysfunction by flow cytometry in chronic lymphocytic leukaemia
Author(s) -
Carter A.,
Lin K.,
Sherrington P. D.,
Pettitt A. R.
Publication year - 2004
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.2004.05223.x
Subject(s) - flow cytometry , blot , ataxia telangiectasia , chronic lymphocytic leukemia , cancer research , mdm2 , mutation , ataxia , medicine , biology , immunology , gene , leukemia , dna damage , genetics , neuroscience , dna
Summary In chronic lymphocytic leukaemia (CLL) cells, functional impairment of the p53 pathway is detectable by Western blotting as impaired up‐regulation of p21 (a transcriptional target of p53) in response to ionizing radiation (IR). The type A defect is characterized by baseline p53 overexpression and is associated with TP53 mutation. The type B defect is characterized by impaired IR‐induced p53 up‐regulation and is associated with inactivation of the ataxia telangiectasia‐mutated gene ( ATM ). Both abnormalities are strongly associated with adverse clinical outcome. In the present study, flow cytometry was found to be an effective alternative to Western blotting in the detection of p53 dysfunction in CLL.