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Development of Duffy transgenic mouse: in vivo expression of human Duffy gene with −33T→C promoter mutation in non‐erythroid tissues
Author(s) -
Chaudhuri Asok,
Yuen Genevieve,
Fang Flora,
Storry Jill
Publication year - 2004
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.2004.05208.x
Subject(s) - biology , genetically modified mouse , transgene , microbiology and biotechnology , phenotype , gene , gata1 , mutation , point mutation , in vivo , gene expression , genetics
Summary Blood group Duffy gene (FY) promoter in Duffy‐negative individuals contains a point mutation in the GATA1 protein‐binding motif, which was suggested to be responsible for erythroid suppression of FY . We developed two transgenic mouse lines with FY from both Duffy phenotypes. Transgenic mice with FY from Duffy‐positive phenotype expressed Duffy protein both in red blood cells (RBCs) and non‐erythroid tissues. Transgenic mice with FY from Duffy‐negative phenotype did not express Duffy protein in RBCs, but it was expressed in non‐erythroid tissues. This is the first in vivo experimental evidence showing the effect of −33T→C promoter mutation on FY expression.