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Measuring T cell immunity to influenza vaccination in children after haemopoietic stem cell transplantation
Author(s) -
Haining W. Nicholas,
Evans John W.,
Seth Nilufer P.,
Callaway G. Derek,
Wucherpfennig Kai W.,
Nadler Lee M.,
Guinan Eva C.
Publication year - 2004
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.2004.05204.x
Subject(s) - stem cell , vaccination , immunology , transplantation , immunity , medicine , immune system , biology , microbiology and biotechnology
Summary Quantitative assessment of immunogen‐specific T cell responses may provide a meaningful surrogate marker of functional immunity in patients following haemopoietic stem cell transplantation (HSCT). We developed a flow‐cytometric assay to quantify antigen‐specific T cell immunity to influenza‐A and studied the T cell response to influenza vaccination in five children, 3–21 months post‐HSCT. All patients showed an increase in influenza‐A‐specific CD4 + immunity following vaccination while none had a detectable IgG response to the vaccine. This assay proved sufficiently sensitive to evaluate changes in T cell memory in immunocompromised individuals and could be used to better characterize post‐HSCT immune reconstitution.