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Haploidentical donor T cells fail to facilitate engraftment but lessen the immune response of host T cells in murine fetal transplantation
Author(s) -
Chen JengChang,
Chang MingLing,
Lee Hanmin,
Muench Marcus O.
Publication year - 2004
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.2004.05040.x
Subject(s) - immunology , immune system , bone marrow , cd8 , transplantation , cytotoxic t cell , t cell , immune tolerance , peritoneal cavity , andrology , spleen , stem cell , biology , medicine , microbiology and biotechnology , in vitro , anatomy , biochemistry
Summary The effects of donor T cells, or their CD8 + subset, on engraftment and tolerance induction in fetal transplantation were evaluated using an F 1 ‐into‐parent mouse‐model that does not permit a graft‐ versus ‐host effect. Gestational day 13 C57BL/6 (H‐2K b ) fetuses were transplanted with B6D2F 1 (H‐2K b/d ) light density bone marrow cells (LDBMC) containing 1–2% T cells, T‐cell depleted bone marrow cells (TDBMC, <0·1% T cells), or TDBMC with enriched CD8 + T cells (CD8). Chimaerism levels in the peripheral blood, spleen and bone marrow were usually below 0·2% in all groups, indicating that T cells do not improve engraftment without a graft‐ versus ‐host effect. A significant, but transient, wave of donor cells was seen in the peripheral blood at 1 month of age in the CD8 and LDBMC groups. Relatively high levels of chimaerism (<17%) were sometimes detected in the peritoneal cavities of recipients. T‐cell tolerance specific to donor cells was evaluated in mixed lymphocyte cultures. The CD8 and LDBMC groups had significantly lower T‐cell responses than untransplanted controls. These findings indicate that in utero transplantation of haploidentical donor CD8 + or CD3 + cells can help to lessen the immune response of host T cells towards donor cells. The persistence of donor cells in the peritoneal cavity also correlated with tolerance induction.