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Improved extension of platelet storage in a polyolefin container with higher oxygen permeability
Author(s) -
Yuasa Takeshi,
Ohto Hitoshi,
Yasunaga Reiko,
Kai Takanori,
Shirahama Noriaki,
Ogata Takashi
Publication year - 2004
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.2004.04994.x
Subject(s) - platelet , chemistry , oxygen , zoology , food science , medicine , biology , organic chemistry
Summary This study evaluated a newly developed polyolefin bag, which has a 50%/m 2 higher oxygen permeability to extend the shelf‐life of platelets. Single‐donor aphaeresis platelets were pooled, separated equally into two bags, PO‐80 (0·8 l capacity) and KBP‐PO (1 l capacity) for control, and stored in plasma for up to 7 d. Platelet biochemical and functional parameters were monitored in bags containing high (4·2 × 10 11 /250 ml/bag, n = 9) and low (2·0 × 10 11 /200 ml/bag, n = 3) concentrations of platelets over the storage period. After 7 d of storage, the PO‐80 bags containing high concentrations of platelets had a better pH (mean pH 6·74) than those stored in KBP‐PO (pH 6·32, P < 0·01); none of the nine PO‐80 bags with a high platelet concentration had a pH below 6·2, compared with four of nine controls ( P < 0·05). Similarly, lactate values were 20·19 mmol/l and 28·09 mmol/l respectively ( P < 0·05). Aerobic metabolism was maintained better with greater O 2 consumption and less lactate generation in high‐platelet concentration PO‐80 bags than in the control bags. A significant difference was also found in pH, pCO 2 and lactate levels between the two bags containing low concentrations of platelets during the 7‐d period. The in vitro characteristics of platelets declined less over 7 d when stored in a higher oxygen permeable container than in a marketed bag.