Premium
Infused CD34 + cell dose, but not tumour cell content of peripheral blood progenitor cell grafts, predicts clinical outcome in patients with diffuse large B‐cell lymphoma and follicular lymphoma grade 3 treated with high‐dose therapy
Author(s) -
Blystad A. K.,
Delabie J.,
Kvaløy S.,
Holte H.,
Vålerhaugen H.,
Ikonomou I.,
Kvalheim G.
Publication year - 2004
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.2004.04951.x
Subject(s) - cd34 , follicular lymphoma , lymphoma , bone marrow , medicine , diffuse large b cell lymphoma , progenitor cell , pathology , stem cell , cancer research , biology , genetics
Summary Previously, we have shown that patients with diffuse large B‐cell lymphoma (DLBCL) transplanted with contaminated bone marrow (BM) generally have a poor outcome. Whether this is also the case when peripheral blood progenitor cell (PBPC) grafts are used is not known. Forty‐three patients with chemosensitive DLBCL or follicular lymphoma grade 3 (FLgr3) were treated with high‐dose therapy (HDT) and autologous stem cell support. Nine patients received purged grafts. Quantitative real‐time polymerase chain reaction (QRT‐PCR) for either the BCL2/IgH translocation or allele specific oligonucleotide (ASO) QRT‐PCR for the immunoglobulin heavy chain ( IgH ) complementarity‐determining region 3 were used. Nine of 25 (36%) PBPC grafts contained tumour cells as tested by QRT‐PCR, including two grafts purged by CD34 + cell enrichment combined with B‐cell depletion. The level of contamination of the PBPC/CD34 + cells ranged from 0 to 8·28%. No relationship could be shown between the total number of tumour cells infused and relapse. Patients receiving PCR‐positive or PCR‐negative PBPC grafts had similar progression‐free survival (PFS) ( P = 0·49). However, a significant difference was seen in PFS and overall survival (OS) for the patients given ≥6·1 × 10 6 CD34 + cells/kg compared with those given <6·1 × 10 6 CD34 + cells/kg ( P = 0·01 and P < 0·05 respectively).