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Molecular rescue of tumour‐specific T cell receptor idiotype from T cell lymphomas
Author(s) -
Reddy Sunil A.,
Levy Ronald
Publication year - 2004
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.2004.04830.x
Subject(s) - t cell receptor , idiotype , clone (java method) , polymerase chain reaction , lymphoma , biology , microbiology and biotechnology , t cell , virology , complementary dna , gene rearrangement , gene , immunology , antibody , monoclonal antibody , genetics , immune system
Summary The T cell receptor (TCR) idiotype on T cell lymphomas can serve as a vaccine target. To clone the relevant genes, 5′ rapid amplification of cDNA ends (RACE) was performed on 13 T cell lymphomas and nine control samples. Two polymerase chain reactions (PCR) were performed for each TCR chain ( α and β ) and the proportion of the clonal TCR sequence over the total number of TCR sequences was calculated. For α , the average proportions were 0·43 vs. 0·05. For β these were 0·44 and 0·04. The TCR was identified in 10 of 13 lymphoma samples.