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Novel FLT3 point mutations within exon 14 found in patients with acute myeloid leukaemia
Author(s) -
Stirewalt Derek L.,
Meshinchi Soheil,
Kussick Steven J.,
Sheets Kayla M.,
PogosovaAgadjanyan Era,
Willman Cheryl L.,
Radich Jerald P.
Publication year - 2004
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.2004.04808.x
Subject(s) - point mutation , exon , single strand conformation polymorphism , polymerase chain reaction , missense mutation , agarose gel electrophoresis , microbiology and biotechnology , biology , mutation , cancer research , genetics , gene
Summary Internal tandem duplications in FLT3 are the most common mutation in acute myeloid leukaemia (AML), with agarose gel electrophoresis of polymerase chain reaction products (PCR/agarose) being the screening method of choice for these mutations. As PCR/agarose screening does not detect small mutations, single‐stranded conformational polymorphism analyses (PCR/SSCP) were used in an attempt to identify previously unrecognized point mutations in FLT3 exons 14 and 15 of 140 AML patients, using newly designed primers that anneal within intron sequences. Novel missense point mutations were found in exon 14, suggesting additional investigations should be performed in AML and other haematopoietic malignancies, using this sensitive technique.