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Are aberrant BCR – ABL transcripts more common than previously thought?
Author(s) -
Wilson G. A.,
Vandenberghe E. A.,
Pollitt R. C.,
Rees D. C.,
Goodeve A. C.,
Peake I. R.,
Reilly J. T.
Publication year - 2000
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.2000.02471.x
Subject(s) - breakpoint cluster region , multiplex , exon , breakpoint , multiplex polymerase chain reaction , abl , microbiology and biotechnology , biology , polymerase chain reaction , philadelphia chromosome , cancer research , medicine , genetics , gene , chromosomal translocation , receptor , tyrosine kinase
We report the use of multiplex polymerase chain reaction (PCR), using 4% polyacrylamide gel electrophoresis (PAGE) for the detection of BCR‐ABL transcripts in Philadelphia‐positive disease. Three out of 50 cases [two out of 37 chronic myeloid leukaemia (CML), one out of 13 acute lymphoblastic leukaemia (ALL)] possessed rare breakpoints; an e19a2 and e13a3 in CML and an e1a3 in the ALL. We suggest that multiplex PCR using 4% PAGE and optimized for smaller transcript detection may lead to a higher detection rate of rare BCR‐ABL breakpoints. Multiplex PCR, however, failed to distinguish e13a2 from e1a3 transcripts. Finally, the presence of e13a3 in CML supports the view that abl exon 2 sequences are unnecessary for the pathogenesis of ‘classic’ CML.