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Vincristine‐induced apoptosis in vivo in peripheral blood mononuclear cells of children with acute lymphoblastic leukaemia (ALL)
Author(s) -
Groninger E.,
de Graaf S. S. N.,
Meeuwsende Boer G. J.,
Sluiter W. J.,
Poppema S.
Publication year - 2000
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.2000.02420.x
Subject(s) - vincristine , peripheral blood mononuclear cell , tunel assay , in vivo , apoptosis , terminal deoxynucleotidyl transferase , medicine , ex vivo , immunology , pathology , chemotherapy , in vitro , biology , immunohistochemistry , cyclophosphamide , biochemistry , microbiology and biotechnology
We conducted a study to demonstrate vincristine‐induced apoptosis in vivo in peripheral blood mononuclear cells of children with newly diagnosed acute lymphoblastic leukaemia (ALL). In five children, apoptosis was detected by terminal deoxynucleotide transferase‐mediated dUTP‐digoxigenin nick‐end labelling (TUNEL) assay in blood samples collected during an up‐front window study of vincristine monotherapy. In one patient, we found a striking increase of apoptotic cells from 2% (SEM 0·6) before to 40·7% (SEM 2·9) 3 h after vincristine injection. In the other patients, the maximum increase ranged from 0·8% to 4·3%. Wilcoxon matched pairs analysis of all patients confirmed that vincristine induces apoptosis in vivo in peripheral blood mononuclear cells in childhood ALL ( P = 0·043).