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Thrombopoietin has a differentiative effect on late‐stage human erythropoiesis
Author(s) -
Liu WenLi,
Wang Min,
Tang Delia C.,
Ding Ivan,
Rodgers Griffin P.
Publication year - 1999
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1999.01343.x
Subject(s) - erythropoiesis , erythropoietin , thrombopoietin , biology , haematopoiesis , progenitor cell , endocrinology , microbiology and biotechnology , medicine , erythropoietin receptor , immunology , stem cell , anemia
To further explore the mechanism of the effect of thrombopoietin (TPO) on erythropoiesis, we used a two‐phase culture system to investigate the effect of TPO on late‐stage human erythroid lineage differentiation. In serum‐free suspension and semisolid cultures of human peripheral blood derived erythroid progenitors, TPO alone did not produce benzidine‐positive cells. However, in serum‐containing culture, TPO alone stimulated erythroid cell proliferation and differentiation, demonstrated by erythroid colony formation, production of benzidine‐positive cells and haemoglobin (Hb) synthesis. Monoclonal anti‐human erythropoietin antibody and anti‐human erythropoietin receptor antibody completely abrogated the erythroid differentiative ability of TPO in the serum‐containing systems. This implied that binding of EPO and EPO‐R was essential for erythropoiesis and the resultant signal transduction may be augmented by the signals emanating from TPO–c‐Mpl interaction. Experiment of withdrawal of TPO further demonstrated the involvement of TPO in late‐stage erythropoiesis. RT‐PCR results showed that there was EPO‐R but not c‐Mpl expression on developing erythroblasts induced by TPO in serum‐containing system. Our results establish that TPO affects not only the proliferation of erythroid progenitors but also the differentiation of erythroid progenitors to mature erythroid cells.