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Expansion and differentiation of haemopoietic progenitor cells on endothelialized hydroxyapatite under static conditions
Author(s) -
Conrad Véronique,
Dupouy Maryse,
Bordenave Laurence,
Lacombe Francis,
Baquey Charles,
Reiffers Josy,
Ripoche Jean
Publication year - 1999
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1999.01286.x
Subject(s) - progenitor cell , megakaryocyte , population , stromal cell , cd34 , microbiology and biotechnology , chemistry , immunology , matrix (chemical analysis) , bone marrow , haematopoiesis , myeloid , andrology , biology , stem cell , medicine , cancer research , environmental health , chromatography
We have analysed the potential of an endothelialized hydroxyapatite matrix (HAP), a synthetic bone substitute, as a cellularized support for the expansion of haemopoietic progenitor cells. Scanning electron microscopy (SEM) showed that the endothelial cells (EC) tended to form a monolayer fitting closely to the matrix, and that the progenitors adhered to the EC layer. Endothelialized HAP supported the proliferation and differentiation of the progenitors with the addition of the exogenous cytokines IL‐1, and IL‐3. The expanded cell population essentially belonged to the myeloid and monocytic lineages, with a smaller percentage of the megakaryocyte lineage. In comparative experiments CD34 + progenitors were expanded on endothelialized tissue culture flasks, and a significant higher viability of the expanded cells was found with the endothelialized HAP. A high percentage (approx. 40 %) of mature granulocytes was generated in accordance with the presence of differentiating cytokines such as G‐CSF and GM‐CSF at high concentrations in the coculture medium. Other cytokines that could be detected were IL‐6, M‐CSF, SCF, flt3‐ligand. More than 50% of the expanded cell population was able to phagocytose bacteria and to generate an oxydative burst. These data indicate that cellularized HAP may be a useful matrix for stromal cell‐based expansion systems.