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Imaging red blood cells with the atomic force microscope
Author(s) -
Zachée Pierre,
Snauwaert Johan,
Vandenberghe Peter,
Hellemans Louis,
Boogaerts Marc
Publication year - 1996
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1996.tb08991.x
Subject(s) - red blood cell , atomic force microscopy , glutaraldehyde , microscope , blood cell , red cell , microscopy , materials science , chemistry , biomedical engineering , pathology , biophysics , nanotechnology , medicine , biology , immunology , biochemistry
Summary. A novel technique for the reproduction of ultramorphological images and details of the surface of normal and pathological red blood cells (RBC) was investigated. The atomic force microscope (AFM) provided high‐resolution images of cell surfaces. The RBC dimensions obtained by this technique revealed differences between native red cells in smears and glutaraldehyde‐fixed red cells. It was shown that fixed red blood cells were best suited for the ultramorphological imaging of the cell surface.