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Iron chelators induce apoptosis in proliferating cells
Author(s) -
Hileti Dona,
Panayiotidis Panayiotis,
Hoffbrand A. Victor
Publication year - 1995
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1995.tb08927.x
Subject(s) - apoptosis , microbiology and biotechnology , cancer research , chemistry , biology , biochemistry
Summary. The iron chelators 1,2‐dimethyl‐3‐hydroxypyrid‐4‐one (L 1 ) and desferrioxamine (DFO) were found to induce apoptosis of proliferating activated T‐lymphocytes and of the promyelocytic cell line HL60, but not of resting peripheral blood lymphocytes or granulocytes. The induction of apoptosis was quantified by propidium iodide staining of apoptotic/dead cells and flow cytometry. In activated T‐lymphocytes incubated with the chelators at equivalent iron‐binding concentrations (300 μM L 1 or 100 μm DFO) for 24 h, L 1 caused a 54% increase in cell death and DFO a 57% increase. In HL60 cells L 1 caused a 50% increase in cell death and DFO a 40% increase. DNA cytofluorometry of HL60 cells treated with either chelator showed an increase in the percentage of cells with hypodiploid DNA content. Presaturation of the chelators with ferric chloride abrogated these effects. L 1 and DFO did not induce apoptosis in resting peripheral blood lymphocytes or granulocytes, even after 48 h of incubation.