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Detection of NPM‐ALK DNA rearrangement in CD30 positive anaplastic large cell lymphoma
Author(s) -
Waggott W.,
Lo Y.M. D.,
Bastard C.,
Gatter K. C.,
Leroux D.,
Mason D. Y.,
Boultwood J.,
Wainscoat J. S.
Publication year - 1995
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1995.tb08434.x
Subject(s) - anaplastic lymphoma kinase , anaplastic large cell lymphoma , chromosomal translocation , nucleophosmin , cd30 , lymphoma , cancer research , fusion gene , dna , gene rearrangement , biology , microbiology and biotechnology , fluorescence in situ hybridization , gene , genetics , chromosome , medicine , tumor cells , oncology , immunology , malignant pleural effusion , lung cancer
CD30 positive anaplastic large cell lymphoma (ALCL) is a type of non‐Hodgkin's lymphoma associated with a specific chromosome translocation between chromosomes 2 and 5. Recent molecular characterization of the translocation breakpoint has identified a gene fusion between NPM (nucleophosmin) and ALK (anaplastic lymphoma kinase). Using a DNA hybridization technique, the NPM rearrangement was found among 5/5 ALCL samples. We have developed a PCT methodology which has enabled the detection of the NPM‐ALK rearrangements amongst seven t(2; 5)(p23; q35) ALCL cases based on a long‐range PCR of genomic DNA. The rapidity and robustness of this method may have diagnostic applications for ALCL.