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Detection of chromosome 11 alterations in blood and bone marrow by interphase cytogenetics in mantle cell lymphoma
Author(s) -
Zucca Emanuele,
Soldati Gianni,
Schlegelberger Brigitte,
Booth Mark J.,
WeberMatthiesen Klaus,
Cavalli Franco,
Cotter Finbarr E.
Publication year - 1995
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1995.tb08386.x
Subject(s) - chromosomal translocation , fluorescence in situ hybridization , biology , mantle cell lymphoma , interphase , cytogenetics , bone marrow , microbiology and biotechnology , pathology , chromosome , lymphoma , genetics , immunology , medicine , gene
The t(11;14)(q13;q32) translocation is a consistent chromosome change in mantle cell lymphomas. This study investigates the application of fluorescent in situ hybridization (FISH) with chromosome painting probes for interphase cytogenetic analysis in patients with mantle cell lymphomas. Chromosome 11 paints have been able to show splitting of the chromosome signal consistent with the t(11; 14) translocation in interphase cells from bone marrow and blood of patients with mantle cell lymphomas. These include some in clinical remission. The chromosome probes conjugated with fluorescent molecules are hybridized with patient's DNA allowing the easy detection of chromosome 11 abnormalities with fluorescent‐light microscopes. Interphase FISH has a higher sensitivity and is quicker than standard metaphase cytogenetics. This may be beneficial in rapid detection of chromosome 11 abnormalities, assisting in the diagnosis of mantle cell lymphomas. In addition, detection of a clonal population of cells is possible.