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Application of a polymorphic Y microsatellite to the detection of post bone marrow transplantation chimaerism
Author(s) -
Lo Y.M. D.,
Noakes L.,
Roux E.,
Jeannet M.,
Chapuis B.,
Fleming K. A.,
Wainscoat J. S.
Publication year - 1995
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1995.tb08380.x
Subject(s) - microsatellite , bone marrow transplantation , transplantation , biology , allele , polymerase chain reaction , dna , bone marrow , genetics , immunology , medicine , gene
A highly sensitive Y‐microsatellite amplification system was developed for the detection of post bone marrow transplantation (BMT) chimaerism. The system is able to detect the DNA equivalent of a single male cell in a background of 10 5 female cells. For clinical applications it has the distinct advantage that the polymorphic nature of the PCR product allows the checking of the genuineness of a positive PCR signal by reference to its allelic type. This extra degree of precision enhances the accuracy of Y‐PCR for clinical diagnosis.