Premium
Flow cytometric analysis of decay‐accelerating factor (CD55) on neutrophils from aplastic anaemia patients
Author(s) -
FORES RAFAEL,
ALCOCER MARIA,
DIEZMARTIN JOSE L.,
FERNANDEZ MANUEL N.
Publication year - 1995
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1995.tb05611.x
Subject(s) - flow cytometry , medicine , aplastic anemia , gastroenterology , immunology , bone marrow
Summary. Using a flow cytometric analysis, CD55 (decay‐accelerating factor), CD59 and CD58 have been measured on neutrophils from 12 aplastic anaemia (AA) patients who were long‐term survivors after immunosuppressive therapy (IS), 17 healthy individuals, four patients with PNH, and six patients with other haematological disorders. The neutrophils from normal control patients and the six patients with other haematological disorders showed 98 ±2% (mean ±SD) positive granulocytes for CD55. Corresponding values were low (12%, 26%, 51% and 58%) on the primarily PNH patients. Among the 12 AA patients examined, seven had normal and five low values (59% in two, 70%, 71% and 82%). Among the five A A patients who showed CD 5 5 neutrophil deficiency, four had showed an incomplete response after the initial IS treatment and the other relapsed following an initial haematological complete response; three cases had a positive Ham's test and two were negative. Our data suggest that the development of PNH clones is a frequent finding in A A long‐term survivors, mainly in those who had shown an incomplete response following IS. Neutrophil CD55 expression analysis by flow cytometry could be useful to detect clonal evolution in these patients.