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Combined immunophenotyping and in situ hybridization (FICTION): a rapid method to study cell lineage involvement in myelodysplastic syndromes
Author(s) -
SOENEN VALERIE,
FENAUX PIERRE,
FLACTIF MARTIAL,
LEPELLEY PASCALE,
LAI JEAN LUC,
COSSON ALAIN,
PREUDHOMME CLAUDE
Publication year - 1995
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1995.tb05604.x
Subject(s) - immunophenotyping , lineage (genetic) , myelodysplastic syndromes , in situ hybridization , cell lineage , medicine , pathology , immunology , biology , bone marrow , genetics , antigen , cellular differentiation , gene , gene expression
Summary. We present a study in which we used a recently described method combining fluorescence in situ hybridization (FISH) and immunophenotyping, i.e. FICTION, to assess the involvement of different cell lineages in myelodysplastic syndrome (MDS) with monosomy 7 (–7), trisomy 8 (+8) or loss of Y chromosome (–Y). Blood or marrow smears or cytocentrifuge preparations were stained both by antibodies to granulocytes (CD 15 ), monocytes (CD 14 ), T lymphocytes (CD 3 ), B lymphocytes (CD 2 o) and by probes specific for chromosomes 7, 8 or Y. Of nine cases of MDS with –7, four with +8 and two with – Y studied, none showed lymphocytic involvement by the chromosome abnormality. In contrast, ‐7,‐1‐8 and – Y were found in granulocytes and monocytes in all patients studied, but they involved a variable proportion of those cells. The partial involvement by –7 and +8 seen in some cases suggests that myelopoi'esis was only partially clonal in those cases, or that the chromosome abnormality was a secondary event in the MDS process. FICTION therefore appears to be a simple and easily reproducible method that can be used for the assessment of lineage involvement in MDS and other haematological malignancies.