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Haemopoietic progenitor cell differentiation: flow cytometric assessment in bone marrow and thymus
Author(s) -
Tjønnfjord Geir E.,
Steen Rita,
Veiby Ole P.,
Morkrid Lars,
Egeland Torstein
Publication year - 1995
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1995.tb05426.x
Subject(s) - progenitor cell , bone marrow , flow cytometry , progenitor , immunology , stem cell , cellular differentiation , medicine , biology , pathology , cancer research , microbiology and biotechnology , genetics , gene
Summary. We have recently shown that expression of any of the lineage‐associated molecules CD2, CD7, CD10, CD19 or CD33 does not ensure lineage‐commitment of CD34 + progenitor cells. Further, normal progenitor cells and leukaemic blast cells have been shown to coexpress molecules associated with more than one haemopoietic lineage. Five‐dimensional flow cytometric analysis of normal bone marrow cells was exploited to investigate the hypothesis of a developmental stage in haemopoiesis comprising CD34 + cells coexpressing CD2, CD5, CD7, CD10, CD19 and CD33 or any combination of these molecules. We report on a subpopulation of CD34 + bone marrow cells constituting < 5% of the CD34 + cells and characterized by extensive coexpression of several molecules associated with the B lymphoid, T lymphoid and myeloid lineages. There is every probability that some cells display the CD34+ CD2 + CD5 + CD7 + CD10 + CD19+ CD33+ phenotype. Studies on postnatal thymocytes suggest that this may be the phenotype or one of a few phenotypes of a candidate thymus‐seeding progenitor cell population. Finally, our findings that CD34 + as well as CD34 + CD5 + thymocytes can be driven into non‐T‐lymphoid differentiation by cytokines, support the notion that the thymus is seeded by uncommitted progenitors.