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A novel factor‐dependent human myelodysplastic cell line, MDS92, contains haemopoietic cells of several lineages
Author(s) -
Tohyama Kaoru,
Tohyama Yumi,
Nakayama Takashi,
Ueda Takanorf,
Nakamura Toru,
Yoshida Yataro
Publication year - 1995
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1995.tb05391.x
Subject(s) - stem cell factor , megakaryocyte , stem cell , thrombopoietin , biology , myeloid , cd34 , cell culture , interleukin 3 , microbiology and biotechnology , progenitor cell , haematopoiesis , immunology , cancer research , t cell , antigen presenting cell , genetics , immune system
Summary. A novel long‐term cultured interleukin(IL)‐3‐dependent human myelodysplastic cell line, MDS92, was shown to contain several myeloid‐lineage cells such as neutrophils, macrophages, eosinophils, and a small number of megakaryocyte‐lineage cells. Therefore this cell line possesses at least bipotential characteristics of myeloid‐ and megakaryocyte‐lineages. Granulocyte colony‐stimulating factor clearly promoted the neutrophil alkaline phosphatase activity of MDS92 cells. To the contrary, the incidence and growth of CD41‐positive cells were hardly affected by the addition of IL‐6, IL‐11, c‐mpl ligand (thrombopoietin, TPO) or erythropoietin. TPO slightly supported the growth of CD34‐positive cell fraction, but not CD41‐positive cell fraction of MDS92 cells in combination with IL‐3 or Steel factor. This cell line will be a useful tool for the study of MDS stem cells, but the mechanism of commitment of differentiation in MDS stem cells remains unknown.