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A simple method for synthesis of B‐cell clonospecific probes
Author(s) -
Saal Russell J.,
Gordon Ross B.,
Cobcroft Ralph G.,
Scott Desley C.
Publication year - 1995
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1995.tb05350.x
Subject(s) - simple (philosophy) , computer science , medicine , computational biology , biology , philosophy , epistemology
Summary. A sensitive PCR‐based method was developed to produce B‐cell clonogenic probes without the need for sequencing and specific oligonucleotide synthesis. Specificity and sensitivity were assessed and found to be comparable to that achieved using established methods. Possible applications include the detection of MRD, bone marrow involvement with lymphoma, and the contamination of autologous bone marrow or peripheral blood progenitor cell harvests with malignant cells carrying IgH rearrangements.