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Correlation between a sandwich ELISA and an in‐vitro bioassay for erythropoietin in human plasma
Author(s) -
Sakata Susumu,
Enoki Yasunori,
Shimizu Satoshi,
Hattori Masatoshi,
Ueda Masatsugu
Publication year - 1995
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1995.tb05347.x
Subject(s) - erythropoietin , bioassay , monoclonal antibody , in vitro , recombinant dna , monoclonal , antibody , immunology , human plasma , microbiology and biotechnology , biology , chemistry , andrology , medicine , endocrinology , biochemistry , chromatography , gene , genetics
Summary. A sandwich‐type enzyme‐linked immunosorbent assay (ELISA) for human erythropoietin (EPO) using two anti‐EPO monoclonal antibodies has been compared with an in‐vitro EPO bioassay based on CFU‐E colony formation in fetal mouse liver cell cultures. In normal subjects and non‐uraemic anaemic patients the plasma EPO values estimated with the ELISA correlated well with those by the bioassay, and also inversely with the values of blood Hb, PCV and RBC counts. Dose‐response curves for plasma and standard recombinant human EPO in the ELISA were parallel to each other. These results further confirm the validity for the ELISA in measuring circulating human EPO.