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The effect of human fit ‐ 3 ligand on committed progenitor cell production from normal, aplastic anaemia and Diamond‐Blackfan anaemia bone marrow
Author(s) -
Scopes John,
Daly Stephen,
Ball Sarah E.,
McGockin Colin P.,
GordonSmith Edward C.,
Gibson Frances M.
Publication year - 1995
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1995.tb05345.x
Subject(s) - progenitor cell , diamond–blackfan anemia , bone marrow , cfu gm , granulocyte , aplastic anemia , immunology , granulocyte macrophage colony stimulating factor , colony forming unit , stimulation , biology , medicine , cytokine , stem cell , biochemistry , microbiology and biotechnology , gene , genetics , ribosome , rna , bacteria
Summary. We investigated the effect of the human ligand for flt‐3 (FL) on the committed progenitor colony formation of normal bone marrow (BM) (n = 9) and BM from four aplastic anaemia (AA) and three Diamond‐Blackfan anaemia (DBA) patients. Methylcellulose committed progenitor cell assays were carried out using FL alone and in combinations with granulocyte‐macrophage colony‐stimulating factor (GM‐CSF). interleukin‐3 (IL‐3) and c‐kit ligand (KL). FL alone had a limited, though significant, effect on the production of granulocyte‐macrophage colony‐forming unit (CFU‐GM) colonies from normal BM and showed an additive effect with IL‐ 3 and GM‐CSF separately, but not in combination. FL did not increase the stimulation of KL and did not have an effect on the production of erythroid progenitor colonies. FL had no effect on the AA and DBA BMs studied.