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CD34 + cell immunoselection from G‐CSF‐alone‐primed peripheral blood in children with low body mass
Author(s) -
Kanold J.,
Berger M.,
Rapatel C.,
Lumley L. de,
Lutz P.,
Plantaz D.,
Vannier J. P.,
Bergeron C.,
Mechinaud F.,
Favrot M.,
Bonhomme J.,
Travade P.,
CHRU F. Demeocq
Publication year - 1995
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1995.tb05318.x
Subject(s) - leukapheresis , melphalan , cd34 , medicine , busulfan , peripheral blood , peripheral , urology , nuclear medicine , chemotherapy , stem cell , biology , cyclophosphamide , genetics
Summary. We report the data of CD34 + cell immunoselection from peripheral blood after G‐CSF‐alone mobilization (lOμg/kg/d s.c.) in nine children with neuroblastoma (median age 4 5 years (2‐8), median body weight 16kg (10‐20). Leukaphereses were carried out on a Cobe Spectra separator and two consecutive harvests (4 blood volumes processed) were used for immunoselection on a Ceprate(tm) column. The yield of CD34 + cells in the purified fraction was 50% (23‐80), with a median number of 2.8xl0 6 CD34+ cells/kg (1‐9‐4). All patients were reinfused with selected CD34 + cells after busulfan 600 mg/m 2 + melphalan 180mg/m 2 and achieved successful haemopoietic recovery.