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Recombinant PDGF enhances megakaryocytopoiesis in vitro
Author(s) -
Yang Mo,
Chesterman Colin N.,
Chong Beng H.
Publication year - 1995
Publication title -
british journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.907
H-Index - 186
eISSN - 1365-2141
pISSN - 0007-1048
DOI - 10.1111/j.1365-2141.1995.tb05291.x
Subject(s) - megakaryocytopoiesis , megakaryocyte , platelet derived growth factor receptor , thrombopoietin , growth factor , biology , colony stimulating factor , interleukin 3 , haematopoiesis , medicine , endocrinology , microbiology and biotechnology , immunology , stem cell , receptor , biochemistry , immune system , t cell , antigen presenting cell
Summary. The effect of recombinant platelet‐derived growth factor (PDGF) on both murine and human megakaryocyte colony formation was studied in the plasma clot culture system. PDGF significantly stimulates megakaryocyte colony formation in a dose‐dependent manner. The minimum concentration which had a significant stimulating effect on colony forming unit megakaryocyte (CFU‐MK) was lOng/ml and maximum stimulation occurred at 50 ng/ml. The effect of PDGF was compared with that of interleukin (IL)‐3, IL‐6, granulocyte‐macrophage colony stimulating factor (GM‐CSF), erythropoietin (EPO) and acid fibroblast growth factor (aFGF) on megakaryocyte colony formation. The results showed that megakaryocyte colony stimulating activity of PDGF was slightly higher than those of GM‐CSF and aFGF, but lower than those of IL‐3, IL‐6 and EPO. The effect of PDGF in combination with IL‐3 or IL‐6 on megakaryocyte colony formation was also investigated. No synergistic action was found between PDGF and IL‐3 or IL‐6, but an additive effect was observed with IL‐3 plus IL‐6. We also studied the effects of PDGF in combination with anti‐IL6, anti‐IL‐3 or anti‐GM‐CSF antibody. The increase of megakaryocyte colony formation induced by PDGF was partially inhibited by anti‐IL‐6 or anti‐GM‐CSF antibody but not by anti‐IL‐3 antibody. These results indicate that PDGF is a positive regulator for megakaryocytopoiesis in vitro and IL‐6 and GM‐CSF may play a role in the mechanism whereby PDGF stimulates megakaryocytopoiesis.